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Lateral view of a Male Baetis (Baetidae) (Blue-Winged Olive) Mayfly Dun from Mystery Creek #43 in New York
Blue-winged Olives
Baetis

Tiny Baetis mayflies are perhaps the most commonly encountered and imitated by anglers on all American trout streams due to their great abundance, widespread distribution, and trout-friendly emergence habits.

Dorsal view of a Setvena wahkeena (Perlodidae) (Wahkeena Springfly) Stonefly Nymph from Mystery Creek #199 in Washington
As far as I can tell, this species has only previously been reported from one site in Oregon along the Columbia gorge. However, the key characteristics are fairly unmistakable in all except for one minor detail:
— 4 small yellow spots on frons visible in photos
— Narrow occipital spinule row curves forward (but doesn’t quite meet on stem of ecdysial suture, as it's supposed to in this species)
— Short spinules on anterior margin of front legs
— Short rposterior row of blunt spinules on abdominal tergae, rather than elongated spinules dorsally
I caught several of these mature nymphs in the fishless, tiny headwaters of a creek high in the Wenatchee Mountains.
27" brown trout, my largest ever. It was the sub-dominant fish in its pool. After this, I hooked the bigger one, but I couldn't land it.
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Lateral view of a Female Isoperla fusca (Perlodidae) (Yellow Sally) Stonefly Adult from the Yakima River in Washington
The family ID on this one was a little bit tricky. Just going by the size, shape, and color, it looks like Chloroperlidae. However, the second anal vein of the forewing is does not appear to be forked, and the apical maxillary palpal segment is close to the length of the penultimate segment, both of which rule out that family. The position of the cubitoanal crossvein relative to the anal cell in the forewing -- touching it in this case -- indicates Perlidae (and it really doesn't have the "look" of Perlidae at all), but other characteristics, such as the metathorastic sternacostal sutures and lack of gill remnants, point to Perlodidae. That's the right answer. Moving on to Perlodidae, the key characteristics in Merritt & Cummins lead straightforwarly to Isoperla, and the species key in Jewett 1959 (The Stoneflies of the Pacific Northwest) leads to Isoperla fusca.

There is one caveat: That source does suggest a May-July emergence, whereas this one was collected in mid-September.
Troutnut
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Troutnut on Sep 19, 2020September 19th, 2020, 7:03 am EDT
See the caption to this specimen for the reasons why I'm not sure what family to place it in. In a nutshell, it looks like Chloroperlidae, seems to key to Perlidae, and doesn't look like Perlidae at all. I'm either missing or misinterpreting some key features or stumbled upon a specimen that violates a bunch of "usually" characteristics in the key.
Jason Neuswanger, Ph.D.
Troutnut and salmonid ecologist
Troutnut
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Bellevue, WA

Posts: 2758
Troutnut on Nov 12, 2020November 12th, 2020, 6:24 am EST
The new microscope cleared this one up for me; I'm fairly confident it's Isoperla, and most likely fusca, although the timing doesn't match the reported emergence times for that species in a 70-year-old source.
Jason Neuswanger, Ph.D.
Troutnut and salmonid ecologist

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