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Lateral view of a Female Hexagenia limbata (Ephemeridae) (Hex) Mayfly Dun from the Namekagon River in Wisconsin
Hex Mayflies
Hexagenia limbata

The famous nocturnal Hex hatch of the Midwest (and a few other lucky locations) stirs to the surface mythically large brown trout that only touch streamers for the rest of the year.

Dorsal view of a Neoleptophlebia (Leptophlebiidae) Mayfly Nymph from the Yakima River in Washington
Some characteristics from the microscope images for the tentative species id: The postero-lateral projections are found only on segment 9, not segment 8. Based on the key in Jacobus et al. (2014), it appears to key to Neoleptophlebia adoptiva or Neoleptophlebia heteronea, same as this specimen with pretty different abdominal markings. However, distinguishing between those calls for comparing the lengths of the second and third segment of the labial palp, and this one (like the other one) only seems to have two segments. So I'm stuck on them both. It's likely that the fact that they're immature nymphs stymies identification in some important way.
27" brown trout, my largest ever. It was the sub-dominant fish in its pool. After this, I hooked the bigger one, but I couldn't land it.
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Ameletus cooki (Brown Dun) Mayfly Nymph Pictures

I caught this Ameletus nymph with several others of the same kind. This was the most vivid example, but they all had quite a bit of striking and unusual red shading, especially on the last few abdominal segments.

I keyed it out under the microscope using Larvae and adults of Ameletus mayflies (Ephemeroptera: Ameletidae) from Alberta with slightly larger (10 mm), mature specimen with darkened wingpads. Microscope pictures are from that specimen. The characteristics in the key and most of the verification table point pretty clearly to Ameletus cooki, except that the coloration of the antennae more closely resembles Ameletus sparsatus. However, on other characteristics in which these species differ (spines on the dorsal surface of the front femora, which seem very short in this specimen; length of posterolateral spines on segments 8–9; length of spines on posterior edge of tergites 6–9), this is a better match for cooki, and that's probably the correct ID.

Ameletus cooki (Ameletidae) (Brown Dun) Mayfly Nymph from the South Fork Snoqualmie River in Washington
Dorsal view of a Ameletus cooki (Ameletidae) (Brown Dun) Mayfly Nymph from the South Fork Snoqualmie River in Washington
Ventral view of a Ameletus cooki (Ameletidae) (Brown Dun) Mayfly Nymph from the South Fork Snoqualmie River in Washington
Ameletus cooki (Ameletidae) (Brown Dun) Mayfly Nymph from the South Fork Snoqualmie River in Washington
Ruler view of a Ameletus cooki (Ameletidae) (Brown Dun) Mayfly Nymph from the South Fork Snoqualmie River in Washington The smallest ruler marks are 1 mm.
Gill on abdominal segment 5

Ameletus cooki (Ameletidae) (Brown Dun) Mayfly Nymph from the South Fork Snoqualmie River in Washington
Gill on segment 5, closeup, removed and laid flat

Ameletus cooki (Ameletidae) (Brown Dun) Mayfly Nymph from the South Fork Snoqualmie River in Washington
Ventral view, posterior margins of sternites 7-8

Ameletus cooki (Ameletidae) (Brown Dun) Mayfly Nymph from the South Fork Snoqualmie River in Washington
Ameletus cooki (Ameletidae) (Brown Dun) Mayfly Nymph from the South Fork Snoqualmie River in Washington
Posterolateral spines on segments 8–9

Ameletus cooki (Ameletidae) (Brown Dun) Mayfly Nymph from the South Fork Snoqualmie River in Washington
Dorsal view

Ameletus cooki (Ameletidae) (Brown Dun) Mayfly Nymph from the South Fork Snoqualmie River in Washington

This mayfly was collected from the South Fork Snoqualmie River in Washington on May 14th, 2022 and added to Troutnut.com by Troutnut on May 16th, 2022.


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Ameletus cooki (Brown Dun) Mayfly Nymph Pictures

Collection details
Location: South Fork Snoqualmie River, Washington
Date: May 14th, 2022
Added to site: May 16th, 2022
Author: Troutnut
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